heart sections with wga (Vector Laboratories)
Structured Review

Heart Sections With Wga, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 242 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/heart sections with wga/product/Vector Laboratories
Average 96 stars, based on 242 article reviews
Images
1) Product Images from "NADPH Oxidase 4 Regulates Inflammation in Ischemic Heart Failure: Role of Soluble Epoxide Hydrolase"
Article Title: NADPH Oxidase 4 Regulates Inflammation in Ischemic Heart Failure: Role of Soluble Epoxide Hydrolase
Journal: Antioxidants & Redox Signaling
doi: 10.1089/ars.2018.7548
Figure Legend Snippet: Cardiac remodeling and macrophage infiltration is reduced in Nox4−/− mice after LAD ligation. WT and Nox4−/− mice hearts were analyzed by immunofluorescence microscopy 2 weeks after sham or from the peri-infarct region after LAD ligation (A) Cardiomyocyte CSA was measured by WGA staining. (B) Capillary density was measured by vWF staining. (C) Cell death was measured in cardiomyocytes by co-staining with TUNEL and MYH7, a cardiomyocyte cell marker. (arrowheads indicate TUNEL+ nuclei). (D) Macrophages were identified by CD68 staining in sham-operated mice and in the border region of the infarct zone of LAD-ligated mice. MYH7 was used to identify the border region. (E) Quantification of cardiomyocyte CSA, (F) quantification of capillary density, (G) quantification of TUNEL+ cells, and (H) quantification of CD68+ cells. Data are the mean ± SEM, n = 9. Scale is 100 μm. *p < 0.05, **p < 0.01, and ****p < 0.0001. CSA, cross-sectional area; MYH7, myosin heavy chain beta; TUNEL, terminal deoxynucleotidyl transferase; vWF, von Willebrand Factor; WGA, wheat germ agglutinin.
Techniques Used: Ligation, Immunofluorescence, Microscopy, Staining, TUNEL Assay, Marker
Figure Legend Snippet: Adverse left ventricular remodeling in human hearts with ICM. ICM hearts (n = 19) were compared with NF hearts (n = 24). (A) Representative images of left ventricular cross-sections were stained with WGA (top panels). Cardiomyocyte cross-sectional area was measured by using Image J (n ∼ 500–600 cells; middle panel). (B) Representative images of left ventricular cross-sections stained with von Willebrand factor to determine capillary density (top panel). Quantification of capillary density (n ∼ 100–125 microscopic fields; middle panel) (C) Representative fluorescent microscopy images of TUNEL-stained left ventricular sections (arrowheads indicate TUNEL+ nuclei; upper panel). Quantification of TUNEL+ nuclei (n ∼ 100–125 cross-sectional areas, middle panel) (D) Representative images of left ventricular cross-sections stained with Verhoeff sirius red (upper panel), and collagen was quantified by using Image J (middle panel). Data are the mean ± SEM. Scale bar is 100 μm. Scatter plots showing correlation between NOX4 protein levels and specific left ventricular remodeling markers in all human heart samples (bottom panels). The regression lines in each graph show the 95% confidence intervals (dotted lines), and R2 values are shown for each plot. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.
Techniques Used: Staining, Microscopy, TUNEL Assay